A RP-HPLC method is used for the quantitative estimation of the ondansetron in the injectable dosage form. Ondansetron hydrochloride is the racemic form of ondansetron and selective blocking agent of the serotonin 5-HT3 receptor type and used in the control of nausea and vomiting. The mobile phase used was a combination of sodium dehydrogenase orthophosphate monohydrate and 1-decanesulphonic acid sodium salt: Acetonitrile (75: 25). The detection was carried out at 216 nm and a flow rate of 1.5 ml/min. The retention time of ondansetron was found to be 22.90 min respectively. The assay of ondansetron was found to be 99.2%. The detector used was an UV detector; the reversed phase column used was ACE C8 (150 x 4.6 mm), 5 μ at ambient temperature. Thus the proposed method can be used for the routine analysis of the ondansetron in the pharmaceutical formulation.
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